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Theoretical and experimental study of charge transfer through DNA: impact of mercury mediated T-Hg-T base pair

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F14%3A00432624" target="_blank" >RIV/68378271:_____/14:00432624 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388963:_____/14:00432624 RIV/00216305:26310/14:PU108952 RIV/00216208:11320/14:10362682 RIV/68407700:21340/14:00363985

  • Result on the web

    <a href="http://dx.doi.org/10.1021/jp501986a" target="_blank" >http://dx.doi.org/10.1021/jp501986a</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1021/jp501986a" target="_blank" >10.1021/jp501986a</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Theoretical and experimental study of charge transfer through DNA: impact of mercury mediated T-Hg-T base pair

  • Original language description

    DNA-Hg complexes may play an important role in sensing of DNA defects or in detecting of Hg presence in the environment. A fundamental way of characterizing DNA-Hg complexes is to study the way how the electric charge is transferred through the molecularchain. The main goal of this contribution was to investigate the impact of a mercury metal cation that links two thymine bases in a DNA T-T mismatched base pair (T-Hg-T) on charge transfer through the DNA molecule. We compared the charge transfer efficiencies in standard DNA, DNA with mismatched T-T base pairs and DNA with T-Hg(II)-T base pair. For this purpose we measured the temperature dependence of steady-state fluorescence and UV-VIS of the DNA molecules. The experimental results were confronted with the results obtained employing theoretical DFT methods. Generally, the efficiency of charge transfer was driven by mercury changing the spatial overlap of bases.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Physical Chemistry B

  • ISSN

    1520-6106

  • e-ISSN

  • Volume of the periodical

    118

  • Issue of the periodical within the volume

    20

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    5374-5381

  • UT code for WoS article

    000336510300010

  • EID of the result in the Scopus database