The structure of metallo-DNA with consecutive T-HgII-T base-pairs ex-plains positive reaction entropy for the metallo-base-pair formation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F14%3A10290954" target="_blank" >RIV/00216208:11320/14:10290954 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gkt1344" target="_blank" >http://dx.doi.org/10.1093/nar/gkt1344</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkt1344" target="_blank" >10.1093/nar/gkt1344</a>

Result language

angličtina

Original language name

The structure of metallo-DNA with consecutive T-HgII-T base-pairs ex-plains positive reaction entropy for the metallo-base-pair formation

Original language description

We have determined the three-dimensional (3D) structure of DNA duplex that includes tandem Hg II-mediated T-T base pairs (thymine-Hg II - thymine, T-Hg II -T) with NMR spectroscopy in solution. This is the first 3D structure of metalloDNA (covalently metallated DNA) composed exclusively of 'NATURAL' bases. The T-Hg II -T base pairs whose chemical structure was determined with the 15 N NMR spectroscopy were well accommodated in a B-form double helix, mimicking normal Watson-Crick base pairs. The Hg atomsaligned along DNA helical axis were shielded from the bulk water. The complete dehydration of Hg atoms inside DNA explained the positive reaction entropy ("S ) for the T-Hg II -T base pair formation. The positive"S value arises owing to the Hg II dehydration, which was approved with the 3D structure. The 3D structure explained extraordinary affinity of thymine towards Hg II and revealed arrangement of T-Hg II-T base pairs in metallo-DNA.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BJ - Thermodynamics

OECD FORD branch

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Project

<a href="/en/project/GAP205%2F10%2F0228" target="_blank" >GAP205/10/0228: Structure and Dynaics of Bioinorganic Complexes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

42

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

6

Pages from-to

4094-4099

UT code for WoS article

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EID of the result in the Scopus database

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