The structure of metallo-DNA with consecutive thymine-HgII-thymine base pairs explains positive entropy for the metallo base pair formation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F13%3A10193443" target="_blank" >RIV/00216208:11320/13:10193443 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gkt1344" target="_blank" >http://dx.doi.org/10.1093/nar/gkt1344</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkt1344" target="_blank" >10.1093/nar/gkt1344</a>

Result language

angličtina

Original language name

The structure of metallo-DNA with consecutive thymine-HgII-thymine base pairs explains positive entropy for the metallo base pair formation

Original language description

We have determined the three-dimensional (3D) structure of DNA duplex that includes tandem HgII-mediated T-T base pairs (thymine-HgII-thymine, T-HgII-T) with NMR spectroscopy in solution. This is the first 3D structure of metallo-DNA (covalently metallated DNA) composed exclusively of 'NATURAL' bases. The T-HgII-T base pairs whose chemical structure was determined with the 15N NMR spectroscopy were well accommodated in a B-form double helix, mimicking normal Watson-Crick base pairs. The Hg atoms alignedalong DNA helical axis were shielded from the bulk water. The complete dehydration of Hg atoms inside DNA explained the positive reaction entropy (?S) for the T-HgII-T base pair formation. The positive ?S value arises owing to the HgII dehydration, which was approved with the 3D structure. The 3D structure explained extraordinary affinity of thymine towards HgII and revealed arrangement of T-HgII-T base pairs in metallo-DNA.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BJ - Thermodynamics

OECD FORD branch

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Project

<a href="/en/project/GAP205%2F10%2F0228" target="_blank" >GAP205/10/0228: Structure and Dynaics of Bioinorganic Complexes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research [online]

ISSN

1362-4962

e-ISSN

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Volume of the periodical

Neuveden

Issue of the periodical within the volume

December 26, 2013

Country of publishing house

TR - TURKEY

Number of pages

6

Pages from-to

1-6

UT code for WoS article

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EID of the result in the Scopus database

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