Termination pattern of nanocrystalline diamond controls cell behavior
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F14%3A00434473" target="_blank" >RIV/68378271:_____/14:00434473 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Termination pattern of nanocrystalline diamond controls cell behavior
Original language description
The aim of the study was to investigate the behavior of human osteoblastic cells (SAOS-2) on nanocrystalline diamond (NCD) film with altered wettability with a focus on primary adhesion. Surface termination and thus wettability of the NCD was achieved byexposition to oxygen or hydrogen MW plasma. Two different surface termination patterns of the NCD were prepared: 1) half of the surface terminated by O and the other half by H, 2) surface with 200 um wide O/H alternating stripes. Differences in cell behavior caused by these various termination patterns were studied by live cell imaging phase contrast microscopy and subsequent image analysis based on image thresholding and cell tracking in NIS Elements software. Using these methods we determined the number of adherent and non-adherent cells and parameters of each cell migration track on the NCD samples with O/H halves and O/H stripes.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
BM - Solid-state physics and magnetism
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů