Multispectral atomic force microscopy-infrared nano-imaging of malaria infected red blood cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F18%3A00501540" target="_blank" >RIV/68378271:_____/18:00501540 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/acs.analchem.7b04318" target="_blank" >http://dx.doi.org/10.1021/acs.analchem.7b04318</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.analchem.7b04318" target="_blank" >10.1021/acs.analchem.7b04318</a>
Alternative languages
Result language
angličtina
Original language name
Multispectral atomic force microscopy-infrared nano-imaging of malaria infected red blood cells
Original language description
Atomic force microscopy-infrared (AFM-IR) spectroscopy is a powerful new technique that can be applied to study molecular composition of cells and tissues at the nanoscale. AFM-IR maps are acquired using a single wavenumber value: they show either the absorbance plotted against a single wavenumber value or a ratio of two absorbance values. Here, we implement multivariate image analysis to generate multivariate AFM-IR maps and use this approach to resolve subcellular structural information in red blood cells infected with Plasmodium falciparum at different stages of development. This was achieved by converting the discrete spectral points into a multispectral line spectrum prior to multivariate image reconstruction. The approach was used to generate compositional maps of subcellular structures in the parasites, including the food vacuole, lipid inclusions, and the nucleus, on the basis of the intensity of hemozoin, hemoglobin, lipid, and DNA IR marker bands, respectively.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10305 - Fluids and plasma physics (including surface physics)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Chemistry
ISSN
0003-2700
e-ISSN
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Volume of the periodical
90
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
3140-3148
UT code for WoS article
000427095700026
EID of the result in the Scopus database
2-s2.0-85043234845