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Cyclic changes in the amide bands within Escherichia coli biofilms monitored using real-time infrared attenuated total reflection spectroscopy (IR-ATR)

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F19%3A00519146" target="_blank" >RIV/68378271:_____/19:00519146 - isvavai.cz</a>

  • Result on the web

    <a href="https://doi.org/10.1177/0003702819829081" target="_blank" >https://doi.org/10.1177/0003702819829081</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1177/0003702819829081" target="_blank" >10.1177/0003702819829081</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Cyclic changes in the amide bands within Escherichia coli biofilms monitored using real-time infrared attenuated total reflection spectroscopy (IR-ATR)

  • Original language description

    Contrary to the planktonic state of bacteria, their biofilm form represents severe complications in areas such as human medicine or food industry due to the increasing resistance against harsh conditions and treatment. In the present study, infrared attenuated total reflection (IR-ATR) spectroscopy has been applied as an analytic tool studying Escherichia coli (E. coli) biofilm formation close to real time. We report on IR spectroscopic investigations on the biofilm formation via ATR waveguides probing the biofilm in the spectral window of 1800–900 cm−1 at dynamic flow conditions, which facilitated monitoring the growth dynamics during several days. Observing this behavior via IR spectroscopy allows revealing these cyclical changes in bottom-layered bacteria within the biofilm under continuous nutrient flow, in molecular detail, and during extended periods for the first time.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10610 - Biophysics

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Applied Spectroscopy

  • ISSN

    0003-7028

  • e-ISSN

  • Volume of the periodical

    73

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    9

  • Pages from-to

    424-432

  • UT code for WoS article

    000464420300007

  • EID of the result in the Scopus database

    2-s2.0-85062011734