Cyclic changes in the amide bands within Escherichia coli biofilms monitored using real-time infrared attenuated total reflection spectroscopy (IR-ATR)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F19%3A00519146" target="_blank" >RIV/68378271:_____/19:00519146 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1177/0003702819829081" target="_blank" >https://doi.org/10.1177/0003702819829081</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1177/0003702819829081" target="_blank" >10.1177/0003702819829081</a>
Alternative languages
Result language
angličtina
Original language name
Cyclic changes in the amide bands within Escherichia coli biofilms monitored using real-time infrared attenuated total reflection spectroscopy (IR-ATR)
Original language description
Contrary to the planktonic state of bacteria, their biofilm form represents severe complications in areas such as human medicine or food industry due to the increasing resistance against harsh conditions and treatment. In the present study, infrared attenuated total reflection (IR-ATR) spectroscopy has been applied as an analytic tool studying Escherichia coli (E. coli) biofilm formation close to real time. We report on IR spectroscopic investigations on the biofilm formation via ATR waveguides probing the biofilm in the spectral window of 1800–900 cm−1 at dynamic flow conditions, which facilitated monitoring the growth dynamics during several days. Observing this behavior via IR spectroscopy allows revealing these cyclical changes in bottom-layered bacteria within the biofilm under continuous nutrient flow, in molecular detail, and during extended periods for the first time.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied Spectroscopy
ISSN
0003-7028
e-ISSN
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Volume of the periodical
73
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
424-432
UT code for WoS article
000464420300007
EID of the result in the Scopus database
2-s2.0-85062011734