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Effects of radiation damage and inelastic scattering on single-particle imaging of hydrated proteins with an X-ray Free-Electron Laser

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F21%3A00546207" target="_blank" >RIV/68378271:_____/21:00546207 - isvavai.cz</a>

  • Alternative codes found

    RIV/61389021:_____/21:00580116

  • Result on the web

    <a href="http://hdl.handle.net/11104/0322754" target="_blank" >http://hdl.handle.net/11104/0322754</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1038/s41598-021-97142-5" target="_blank" >10.1038/s41598-021-97142-5</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Effects of radiation damage and inelastic scattering on single-particle imaging of hydrated proteins with an X-ray Free-Electron Laser

  • Original language description

    We present a computational case study of X-ray single-particle imaging of hydrated proteins on an example of 2-Nitrogenase–Iron protein covered with water layers of various thickness, using a start-to-end simulation platform and experimental parameters of the SPB/SFX instrument at the European X-ray Free-Electron Laser facility. The simulations identify an optimal thickness of the water layer at which the efective resolution for imaging the hydrated sample becomes signifcantly higher than for the non-hydrated sample. This efect is lost when the water layer becomes too thick. Even though the detailed results presented pertain to the specifc sample studied, the trends which we identify should also hold in a general case. We expect these fndings will guide future single-particle imaging experiments using hydrated proteins.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10306 - Optics (including laser optics and quantum optics)

Result continuities

  • Project

    <a href="/en/project/LTT17015" target="_blank" >LTT17015: Reserch in frame International Center for Dense Magnetized Plasmas</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Scientific Reports

  • ISSN

    2045-2322

  • e-ISSN

    2045-2322

  • Volume of the periodical

    11

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    11

  • Pages from-to

    17976

  • UT code for WoS article

    000695272000099

  • EID of the result in the Scopus database

    2-s2.0-85114771514