Effects of radiation damage and inelastic scattering on single-particle imaging of hydrated proteins with an X-ray Free-Electron Laser
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F21%3A00546207" target="_blank" >RIV/68378271:_____/21:00546207 - isvavai.cz</a>
Alternative codes found
RIV/61389021:_____/21:00580116
Result on the web
<a href="http://hdl.handle.net/11104/0322754" target="_blank" >http://hdl.handle.net/11104/0322754</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-021-97142-5" target="_blank" >10.1038/s41598-021-97142-5</a>
Alternative languages
Result language
angličtina
Original language name
Effects of radiation damage and inelastic scattering on single-particle imaging of hydrated proteins with an X-ray Free-Electron Laser
Original language description
We present a computational case study of X-ray single-particle imaging of hydrated proteins on an example of 2-Nitrogenase–Iron protein covered with water layers of various thickness, using a start-to-end simulation platform and experimental parameters of the SPB/SFX instrument at the European X-ray Free-Electron Laser facility. The simulations identify an optimal thickness of the water layer at which the efective resolution for imaging the hydrated sample becomes signifcantly higher than for the non-hydrated sample. This efect is lost when the water layer becomes too thick. Even though the detailed results presented pertain to the specifc sample studied, the trends which we identify should also hold in a general case. We expect these fndings will guide future single-particle imaging experiments using hydrated proteins.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)
Result continuities
Project
<a href="/en/project/LTT17015" target="_blank" >LTT17015: Reserch in frame International Center for Dense Magnetized Plasmas</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
11
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
17976
UT code for WoS article
000695272000099
EID of the result in the Scopus database
2-s2.0-85114771514