In Situ monitoring of rolling circle amplification on a solid support by surface plasmon resonance and optical waveguide spectroscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F21%3A00552380" target="_blank" >RIV/68378271:_____/21:00552380 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1021/acsami.1c03715" target="_blank" >https://doi.org/10.1021/acsami.1c03715</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsami.1c03715" target="_blank" >10.1021/acsami.1c03715</a>
Alternative languages
Result language
angličtina
Original language name
In Situ monitoring of rolling circle amplification on a solid support by surface plasmon resonance and optical waveguide spectroscopy
Original language description
The growth of surface-attached single-stranded deoxyribonucleic acid (ssDNA) chains is monitored in situ using an evanescent wave optical biosensor that combines surface plasmon resonance (SPR) and optical waveguide spectroscopy (OWS). The “grafting-from” growth of ssDNA chains is facilitated by rolling circle amplification (RCA), and the gradual prolongation of ssDNA chains anchored to a gold sensor surface is optically tracked in time. At a sufficient density of the polymer chains, the ssDNA takes on a brush architecture with a thickness exceeding 10 μm, supporting a spectrum of guided optical waves traveling along the metallic sensor surface. The simultaneous probing of this interface with the confined optical field of surface plasmons and additional more delocalized dielectric optical waveguide modes enables accurate in situ measurement of the ssDNA brush thickness, polymer volume content, and density gradients.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
<a href="/en/project/EF18_053%2F0016627" target="_blank" >EF18_053/0016627: FZU researchers, technical and administrative staff mobility</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Applied Materials and Interfaces
ISSN
1944-8244
e-ISSN
1944-8252
Volume of the periodical
13
Issue of the periodical within the volume
27
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
32352-32362
UT code for WoS article
000674333400101
EID of the result in the Scopus database
2-s2.0-85110932574