Rolling circle amplification tailored for plasmonic biosensors: from ensemble to single-molecule detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F22%3A00566261" target="_blank" >RIV/68378271:_____/22:00566261 - isvavai.cz</a>
Result on the web
<a href="https://hdl.handle.net/11104/0337647" target="_blank" >https://hdl.handle.net/11104/0337647</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsami.2c14500" target="_blank" >10.1021/acsami.2c14500</a>
Alternative languages
Result language
angličtina
Original language name
Rolling circle amplification tailored for plasmonic biosensors: from ensemble to single-molecule detection
Original language description
We report on the tailoring of rolling circle amplification (RCA) for affinity biosensors relying on the optical probing of their surface with confined surface plasmon field. Affinity capture of the target analyte at the metallic sensor surface (e.g., by using immunoassays) is followed by the RCA step for subsequent readout based on increased refractive index (surface plasmon resonance, SPR) or RCA-incorporated high number of fluorophores (in surface plasmon-enhanced fluorescence, PEF). By combining SPR and PEF methods, this work investigates the impact of the conformation of long RCA-generated single-stranded DNA (ssDNA) chains to the plasmonic sensor response enhancement. In order to confine the RCA reaction within the evanescent surface plasmon field and hence maximize the sensor response, an interface carrying analyte-capturing molecules and additional guiding ssDNA strands (complementary to the repeating segments of RCA-generated chains) is developed.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Applied Materials and Interfaces
ISSN
1944-8244
e-ISSN
1944-8252
Volume of the periodical
14
Issue of the periodical within the volume
49
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
55017-55027
UT code for WoS article
000891815800001
EID of the result in the Scopus database
2-s2.0-85143513040