DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F21%3A00566160" target="_blank" >RIV/68378271:_____/21:00566160 - isvavai.cz</a>
Result on the web
<a href="http://doi.org/10.1080/09553002.2021.1948140" target="_blank" >http://doi.org/10.1080/09553002.2021.1948140</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/09553002.2021.1948140" target="_blank" >10.1080/09553002.2021.1948140</a>
Alternative languages
Result language
angličtina
Original language name
DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time
Original language description
Purpose The complex relationship between linear energy transfer (LET) and cellular response to radiation is not yet fully elucidated. To better characterize DNA damage after irradiations with therapeutic protons, we monitored formation and disappearance of DNA double-strand breaks (DNA DSB) as a function of LET and time. Comparisons with conventional gamma-rays and high LET carbon ions were also performed. Materials and Methods In the present work, we performed immunofluorescence-based assay to determine the amount of DNA DSB induced by different LET values along the 62 MeV therapeutic proton Spread out Bragg peak (SOBP) in three cancer cell lines, i.e. HTB140 melanoma, MCF-7 breast adenocarcinoma and HTB177 non-small lung cancer cells. Time dependence of foci formation was followed as well. To determine irradiation positions, corresponding to the desired LET values, numerical simulations were carried out using Geant4 toolkit.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10303 - Particles and field physics
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Radiation Biology
ISSN
0955-3002
e-ISSN
1362-3095
Volume of the periodical
97
Issue of the periodical within the volume
9
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
1229-1240
UT code for WoS article
000674807500001
EID of the result in the Scopus database
2-s2.0-85110868328