Development of the Pseudomonas syringae pv. morsprunorum Biofilm Monitored in Real Time Using Attenuated Total Reflection Fourier Transform Infrared Measurements in a Flow Cell Chamber
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F23%3A00572880" target="_blank" >RIV/68378271:_____/23:00572880 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/23:10465901
Result on the web
<a href="https://doi.org/10.1177/00037028231165057" target="_blank" >https://doi.org/10.1177/00037028231165057</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1177/00037028231165057" target="_blank" >10.1177/00037028231165057</a>
Alternative languages
Result language
angličtina
Original language name
Development of the Pseudomonas syringae pv. morsprunorum Biofilm Monitored in Real Time Using Attenuated Total Reflection Fourier Transform Infrared Measurements in a Flow Cell Chamber
Original language description
BIn the present study, infrared attenuated total reflection spectroscopy coupled with optical and confocal laser scanning microscopy has been applied for the first time to analyze Pseudomonas syringae pathovar morsprunorum biofilm development in real time. The biofilm development was observed within a spectral window 4000–800 cm−1 under constant flow conditions for 72 h. The kinetics of representative integrated band areas (nucleic acids with polysaccharides at 1141–1006 cm−1, amino acid side chains with free fatty acids at 1420–1380 cm−1, proteins at 1580–1490 cm−1, and lipids with proteins at 2935–2915 cm−1) were analyzed with regard to the observed biofilm structure and the following P. syringae biofilm developmental stages were attributed: The inoculation phase, washing of weakly attached bacteria closely followed by recolonization of the vacated surface, the restructuration phase, and finally the maturation phase.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied Spectroscopy
ISSN
0003-7028
e-ISSN
1943-3530
Volume of the periodical
77
Issue of the periodical within the volume
5
Country of publishing house
GB - UNITED KINGDOM
Number of pages
26
Pages from-to
500-512
UT code for WoS article
000976875300001
EID of the result in the Scopus database
2-s2.0-85153745281