Effects of storage on stability and performance of carboxybetaine-based polymer brushes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F24%3A00599586" target="_blank" >RIV/68378271:_____/24:00599586 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11320/24:10494002

Result on the web

<a href="https://www.spiedigitallibrary.org/conference-proceedings-of-spie/12999/3017613/Effects-of-storage-on-stability-and-performance-of-carboxybetaine-based/10.1117/12.3017613.short" target="_blank" >https://www.spiedigitallibrary.org/conference-proceedings-of-spie/12999/3017613/Effects-of-storage-on-stability-and-performance-of-carboxybetaine-based/10.1117/12.3017613.short</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.3017613" target="_blank" >10.1117/12.3017613</a>

Result language

angličtina

Original language name

Effects of storage on stability and performance of carboxybetaine-based polymer brushes

Original language description

Zwitterionic poly(carboxybetaine) brushes have emerged as promising surface functional coatings for antifouling label-free biosensing in complex biological fluids. This study investigates the effect of storage conditions on highly-dense poly(carboxybetaine methacrylamide) (pCBMAA), poly(sulfobetaine methacrylamide) (pSBMAA), a random copolymer of CBMAA with poly(hydroxypropyl methacrylamide) p(CBMAA-HPMAA), and terpolymer p(CBMAA-SBMAA-HPMAA) brushes. Synthesized via surface-initiated atom transfer radical polymerization (SI ATRP) on gold-coated substrates, these brushes were stored in dry, water, or phosphate-buffered saline (PBS) environments at temperatures of 22 °C, 6 °C, or frozen at –20 °C for 44 days. Structural stability of the brushes was assessed using infrared reflection absorption spectroscopy (IRRAS) and spectroscopic ellipsometry (SE), while fouling resistance and IgG antibody binding capacity were evaluated using surface plasmon resonance (SPR). Although the brushes exhibited minimal detachment from the surface, a slight decrease in swelling ratio was observed across all storage conditions. Furthermore, the storage led to a minor reduction in IgG binding capacity, with freezing at –20 °C showing a more pronounced effect in most cases. Notably, storing all tested carboxybetaine-based brushes in water or in PBS at 6 °C adversely affected fouling, a phenomenon alleviated when stored frozen at –20 °C or in a dry state.

Czech name

—

Czech description

—

Type

D - Article in proceedings

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of SPIE

ISBN

978-151067316-8

ISSN

0277-786X

e-ISSN

—

Number of pages

6

Pages from-to

129990O

Publisher name

SPIE

Place of publication

Bellingham

Event location

Strasbourg

Event date

Apr 7, 2024

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

001281471700019