Optical fibre long-period grating sensors modified with antifouling bio-functional nano-brush

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F25%3A00616685" target="_blank" >RIV/68378271:_____/25:00616685 - isvavai.cz</a>

Result on the web

<a href="https://hdl.handle.net/11104/0363669" target="_blank" >https://hdl.handle.net/11104/0363669</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/D4BM01447B" target="_blank" >10.1039/D4BM01447B</a>

Result language

angličtina

Original language name

Optical fibre long-period grating sensors modified with antifouling bio-functional nano-brush

Original language description

Recent advances in optical sensing technologies underpin the development of high-performance, surface-sensitive analytical tools capable of reliable and precise detection of molecular targets in complex biological media in non-laboratory settings. Optical fibre sensors guide light to and from a region of interest, enabling sensitive measurements of localized environments. This positions optical fibre sensors as a highly promising technology for a wide range of biochemical and healthcare applications. However, their performance in real-world biological media is often limited by the absence of robust post-modification strategies that provide both high biorecognition and antifouling capabilities. In this study, we present the proof-of-concept antifouling and biorecognition performance of a polymer brush nano-coating synthesized at the sensing region of optical fibre long-period grating (LPG) sensors. Using a newly developed antifouling terpolymer brush (ATB) composed of carboxybetaine methacrylamide, sulfobetaine methacrylamide, and N (2-hydroxypropyl) methacrylamide, we achieve state-of-the-art antifouling properties. The successful on-fibre ATB synthesis is confirmed through scanning electron microscopy (SEM), fluorescence microscopy, and label free bio-detection experiments based on antibody-functionalized ATB-coated LPG optical fibres. Despite challenges in handling optical fibres during polymerization, the resulting nano-coating retains its remarkable antifouling properties upon exposure to blood plasma and enables biorecognition element functionalization. These capabilities are demonstrated through the detection of IgG in buffer and diluted blood plasma using anti-IgG-functionalized ATB-coated sensing regions of LPG fibres in both label-based (fluorescence) and label free real-time detection experiments. The results show the potential of ATB-coated LPG fibres for use in analytical biosensing applications.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomaterials Science

ISSN

2047-4830

e-ISSN

2047-4849

Volume of the periodical

13

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

1199-1208

UT code for WoS article

001382037400001

EID of the result in the Scopus database

2-s2.0-85212754915