New copolymer brushes for label-free affinity biosensors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F15%3A00462552" target="_blank" >RIV/61389013:_____/15:00462552 - isvavai.cz</a>
Alternative codes found
RIV/67985882:_____/15:00462552
Result on the web
<a href="http://dx.doi.org/10.1109/ICSENS.2015.7370596" target="_blank" >http://dx.doi.org/10.1109/ICSENS.2015.7370596</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1109/ICSENS.2015.7370596" target="_blank" >10.1109/ICSENS.2015.7370596</a>
Alternative languages
Result language
angličtina
Original language name
New copolymer brushes for label-free affinity biosensors
Original language description
Brushes of poly(HPMA-co-CBMAA) copolymer with an adjustable content of functional carboxybetaine (CBMAA) monomer units were grafted from surface of SPR chips using surface initiated atom transfer radical polymerization (SI ATRP). Compared to brushes of of poly(carboxybetaine) (polyCBAA), which so far have provided the best antifouling biorecognition layers, the new brushes were more resistant to undiluted human blood plasma, milk, and crude food extracts, particularly after the covalent attachment of bioreceptors. The incorporation of a selected number of functional CBMAA units in poly(hydroxypropyl methacrylamide) (poly(HPMA)) allowed us to reach ultralow fouling properties better than that of polyCBAA. A typical food-born pathogen bacteria, E.coli O157:H7, was detected in crude extracts of hamburger and lettuce using SPR biosensor coated by biorecognition layers composed of poly(HPMA-co-CBMAA) brushes with covalently attached anti-E. coli antibodies. The SPR biosensor with attached surface antigen of hepatitis B virus HBsAg was used to detect the presence of anti-HBsAg antibodies as biomarkers of hepatitis B infection in standard clinical samples of sera taken from several individuals. The new ultralow fouling biorecognition layers open a feasible way to label-free biosensors capable of in real time detecting analytes in real samples of complex biological media tested for medical diagnostics or food control.
Czech name
—
Czech description
—
Classification
Type
D - Article in proceedings
CEP classification
BO - Biophysics
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GBP205%2F12%2FG118" target="_blank" >GBP205/12/G118: Nanobiophotonics for future health care</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
IEEE SENSORS 2015
ISBN
978-1-4799-8203-5
ISSN
1930-0395
e-ISSN
—
Number of pages
4
Pages from-to
1626-1629
Publisher name
IEEE
Place of publication
New York
Event location
Busan
Event date
Nov 1, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000380440800424