Hepatitis B plasmonic biosensor for the analysis of clinical serum samples
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F16%3A00459400" target="_blank" >RIV/61389013:_____/16:00459400 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.bios.2016.05.014" target="_blank" >http://dx.doi.org/10.1016/j.bios.2016.05.014</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bios.2016.05.014" target="_blank" >10.1016/j.bios.2016.05.014</a>
Alternative languages
Result language
angličtina
Original language name
Hepatitis B plasmonic biosensor for the analysis of clinical serum samples
Original language description
A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10 min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biosensors and Bioelectronics
ISSN
0956-5663
e-ISSN
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Volume of the periodical
85
Issue of the periodical within the volume
15 November
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
272-279
UT code for WoS article
000382410100036
EID of the result in the Scopus database
2-s2.0-84966283583