Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985882%3A_____%2F16%3A00469628" target="_blank" >RIV/67985882:_____/16:00469628 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/16:10327964 RIV/00064165:_____/16:10327964

Result on the web

<a href="http://dx.doi.org/10.1007/s00216-016-9664-z" target="_blank" >http://dx.doi.org/10.1007/s00216-016-9664-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-016-9664-z" target="_blank" >10.1007/s00216-016-9664-z</a>

Result language

angličtina

Original language name

Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples

Original language description

Pregnancy associated plasma protein A2 (PAPP-A2) is a metalloproteinase that plays multiple roles in fetal development and post-natal growth. Here we present a novel surface plasmon resonance (SPR) biosensor for the rapid and quantitative detection of PAPP-A2 in blood samples. This biosensor uses a single surface referencing approach and a sandwich assay with functionalized gold nanoparticles for signal enhancement. We demonstrate that this SPR biosensor enables the detection of PAPP-A2 in 30 % blood plasma at levels as low as 3.6 ng/mL. We also characterize the performance of the biosensor and evaluate its cross-reactivity to a PAPP-A analogue. Finally, we utilize this SPR biosensor for the detection of PAPP-A2 in blood serum from two groups of subjects: pregnant women and healthy non-pregnant women and men

Czech name

—

Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

—

Project

<a href="/en/project/GBP205%2F12%2FG118" target="_blank" >GBP205/12/G118: Nanobiophotonics for future health care</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

—

Volume of the periodical

408

Issue of the periodical within the volume

26

Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

7265-7269

UT code for WoS article

000385155200007

EID of the result in the Scopus database

2-s2.0-84974824534