Osteoblasts win the race for the surface on DNA polyelectrolyte multilayer coatings against S. epidermidis but not against S. aureus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378271%3A_____%2F25%3A00618663" target="_blank" >RIV/68378271:_____/25:00618663 - isvavai.cz</a>
Result on the web
<a href="https://hdl.handle.net/11104/0365514" target="_blank" >https://hdl.handle.net/11104/0365514</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.colsurfb.2024.114336" target="_blank" >10.1016/j.colsurfb.2024.114336</a>
Alternative languages
Result language
angličtina
Original language name
Osteoblasts win the race for the surface on DNA polyelectrolyte multilayer coatings against S. epidermidis but not against S. aureus
Original language description
Co-culture of staphylococcus (S. epidermidis and S. aureus) and SaOS-2 osteosarcoma cells was studied on chitosan-DNA polyelectrolyte multilayer coated glass based on the concept of “the race for the surface.” Staphylococcus was first deposited onto the surface in a microfluidic chamber to mimic peri-operative contamination, SaOS-2 cells were seeded. Both were cultured together on the surfaces for 24 h under flow. The presence of S. epidermidis decreased SaOS-2 cell number on all surfaces after 24 h. The cells that adhered spread equally well in the presence of low virulent S. epidermidis. Highly virulent S. aureus induced cell death of all adherent SaOS-2 cells on chitosan-DNA multilayer coated glass, a worse outcome than on uncoated glass. The outcome of our study demonstrates the need for in vitro co-culture assays to meaningfully bridge the gap in lab testing of biomaterials and their clinical evaluations where bacterial infection can occur.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10610 - Biophysics
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Colloids and Surfaces B-Biointerfaces
ISSN
0927-7765
e-ISSN
1873-4367
Volume of the periodical
245
Issue of the periodical within the volume
Jan
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
114336
UT code for WoS article
001348558800001
EID of the result in the Scopus database
2-s2.0-85207651373