Diamond thin film as functional layer in SGFET and QCM bio-sensors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21110%2F18%3A00323995" target="_blank" >RIV/68407700:21110/18:00323995 - isvavai.cz</a>
Alternative codes found
RIV/68407700:21230/18:00323995
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Diamond thin film as functional layer in SGFET and QCM bio-sensors
Original language description
Recognition of biological or biochemical events using semiconductor devices has been used in many applications. Amongst commonly used materials, a diamond thin film is considered as a promising material for such devices. In this work, we have involved the diamond thin film as a functional layer to recognize different buffer or cultivation solutions. Firstly, we used the hydrogen-terminated diamond film as an optically transparent and electrically conductive channel in solution-gated field effect transistors (SGFET) [1]. We found that loading of the H-diamond channel with buffer solutions (Phosphate PBS, HEPES, and McIlvaine) at equal pH of 7.4 caused diverse voltage shifts in transfer transistor characteristics. The gate voltage shifts after applied different buffers were approx. 50 mV. Repeated measurements with different solutions confirmed the sensing reversibility of the diamond SGFETs. Only the relative value of the voltage (referred to the voltage measured at the first application of sensor) tended to decrease. Secondly, we employed the oxygen or hydrogen terminated diamond coating for the quartz crystal microbalance piezoelectric mass sensor (i.e. H-QCM or O-QCM) [2]. The highest serial resonant frequency (SRF) shifts of 418 and 643 Hz were observed for FBS proteins adsorbed on both sides of O-QCM and H-QCM, respectively. Adsorbed BSA and FN proteins resulted in lower frequency shifts. For FN proteins, the O-QCMs (hydrophilic surface) revealed higher sensitivity than the H-QCMs (hydrophobic surface) as represented by the SRF shit values of 154 and 50 Hz, respectively. This difference is attributed to possible geometrical re-arrangement of molecules adhered on differently terminated diamond surfaces. The work was supported by Czech Science Foundation grant no. P108/12/G108 and Czech Technical University grant no. SGS17/136/OHK4/2T/13.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů