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PDMS substrate stiffness affects the morphology and growth profiles of cancerous prostate and melanoma cells

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F15%3A00221130" target="_blank" >RIV/68407700:21220/15:00221130 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.sciencedirect.com/science/article/pii/S175161611400304X#" target="_blank" >http://www.sciencedirect.com/science/article/pii/S175161611400304X#</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.jmbbm.2014.09.020" target="_blank" >10.1016/j.jmbbm.2014.09.020</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    PDMS substrate stiffness affects the morphology and growth profiles of cancerous prostate and melanoma cells

  • Original language description

    A deep understanding of the interaction between cancerous cells and surfaces is particularly important for the design of lab-on-chip devices involving the use of poly-dimethylsiloxane (PDMS). In our studies, the effect of PDMS substrate stiffness on mechanical properties of cancerous cells was investigated in conditions where the PDMS substrate is not covered with any of extracellular matrix proteins. Two human prostate cancer (Du145 and PC-3) and two melanoma (WM115 and WM266-4) cell lines were cultured on two groups of PDMS substrates that were characterized by istinct stiffness, i.e. 0.75MPa and 2.92 MPa. The results showed the strong effect on cellular behavior and orphology. The detailed analysis of chemical and physical properties of substrates revealed that cellular behavior occurs only due to substrate elasticity.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/TA01010185" target="_blank" >TA01010185: New materials and coatings for joint replacement bionical design</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of the Mechanical Behavior of Biomedical Materials

  • ISSN

    1751-6161

  • e-ISSN

  • Volume of the periodical

    41

  • Issue of the periodical within the volume

    January

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    10

  • Pages from-to

    13-22

  • UT code for WoS article

    000346217400002

  • EID of the result in the Scopus database

    2-s2.0-84908301569