Physico-chemical properties of PDMS surfaces suitable as substratesfor cell cultures
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21220%2F16%3A00300557" target="_blank" >RIV/68407700:21220/16:00300557 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0169433216314362" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0169433216314362</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.apsusc.2016.07.009" target="_blank" >10.1016/j.apsusc.2016.07.009</a>
Alternative languages
Result language
angličtina
Original language name
Physico-chemical properties of PDMS surfaces suitable as substratesfor cell cultures
Original language description
Elastic properties of the substrate have profound effect on adhesion and proliferation of cells. Here, weintroduce a method to produce polydimethylsiloxane (PDMS) substrates with stiffness tuned mono-tonically from 1.67 to 0.24 MPa, by the time of UV irradiation adjusted up to 5 h. The Young’s modulus(determined by using nanoindenter) scales linearly with stiffness calculated using AFM-based force spec-troscopy data. Such a relation enables the determination of the Young modulus from AFM force – distancecurves also when the Herz model is not applicable. Our findings demonstrate that surface propertiesof PDMS substrates are not affected by the applied methodology of tuning substrate elasticity. Finally,the colorimetric proliferation assay (MTT) carried out for non-malignant (HCV29) and cancerous (T24)bladder cancer cells depicted a significant contribution of PDMS substrate elasticity to the behavior ofcells. The softer PDMS substrate demonstrated excellent cytocompatibility whereas the stiff one is morecell-repellent.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied Surface Science
ISSN
0169-4332
e-ISSN
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Volume of the periodical
389
Issue of the periodical within the volume
December
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
247-254
UT code for WoS article
000384577600030
EID of the result in the Scopus database
2-s2.0-84979543891