Optical spectral analysis of ultra-weak photon emission from tissue culture and yeast cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F15%3A00225051" target="_blank" >RIV/68407700:21230/15:00225051 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/15:00457605 RIV/67985882:_____/15:00457605
Result on the web
<a href="http://dx.doi.org/10.1117/12.2069897" target="_blank" >http://dx.doi.org/10.1117/12.2069897</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1117/12.2069897" target="_blank" >10.1117/12.2069897</a>
Alternative languages
Result language
angličtina
Original language name
Optical spectral analysis of ultra-weak photon emission from tissue culture and yeast cells
Original language description
Optical spectral analysis of the ultra-weak photon emission (UPE) could be utilized for non-invasive diagnostic of state of biological systems and for elucidation of underlying mechanisms of UPE generation. Optical spectra of UPE from differentiated HL-60 cells and yeast cells (Saccharomyces cerevisiae) were investigated. Induced photon emission of neutrophil-like cells and spontaneous photon emission of yeast cells were measured using highly sensitive photomultiplier module Hamamatsu H7360-01 in a thermally regulated light-tight chamber. The respiratory burst of neutrophil-like HL-60 cells was induced with the PMA (phorbol 12-myristate, 13-acetate). PMA activates an assembly of NADPH oxidase, which induces a rapid formation of reactive oxygen species(ROS). Long-pass edge filters (wavelength 350, from 400 to 600 with 25 nm resolution and 650 nm) were used for optical spectral analysis. Propagation of error of indirect measurements and standard deviation were used to assess reliability
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA13-29294S" target="_blank" >GA13-29294S: Photonic biosignals: measurement and characterization</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Proc. SPIE 9450, Photonics, Devices, and Systems VI
ISBN
978-1-62841-566-7
ISSN
0277-786X
e-ISSN
—
Number of pages
7
Pages from-to
"94500O-1"-"94500O-7"
Publisher name
SPIE
Place of publication
Bellingham
Event location
Prague
Event date
Aug 27, 2014
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000349404500023