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Super-resolution fluorescence microscopy by line-scanning with an unmodified two-photon microscope

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F21%3A00345383" target="_blank" >RIV/68407700:21230/21:00345383 - isvavai.cz</a>

  • Result on the web

    <a href="https://doi.org/10.1098/rsta.2020.0300" target="_blank" >https://doi.org/10.1098/rsta.2020.0300</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1098/rsta.2020.0300" target="_blank" >10.1098/rsta.2020.0300</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Super-resolution fluorescence microscopy by line-scanning with an unmodified two-photon microscope

  • Original language description

    Fluorescence-based microscopy as one of the standard tools in biomedical research benefits more and more from super-resolution methods, which offer enhanced spatial resolution allowing insights into new biological processes. A typical drawback of utilizing these methods is the need for new, complex optical setups. This becomes even more significant when using two-photon fluorescence excitation, which offers deep tissue imaging and excellent z-sectioning. We show that the generation of striped illumination patterns in twophoton laser scanning microscopy can readily be exploited for achieving optical super-resolution and contrast enhancement utilizing open-source image reconstruction software. The special appeal of this approach is that even in the case of a commercial two-photon laser scanning microscope no optomechanical modifications are required to achieve this modality. Modifying the scanning software with a custom-written macro to address the scanning mirrors in combination with rapid intensity switching by an electro-optic modulator is sufficient to accomplish the acquisition of two-photon striped-illumination patterns on a sCMOS camera. We demonstrate and analyse the resulting resolution improvement by applying different recently published image resolution evaluation procedures to the reconstructed filtered widefield and super-resolved images.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10306 - Optics (including laser optics and quantum optics)

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences

  • ISSN

    1364-503X

  • e-ISSN

    1471-2962

  • Volume of the periodical

    379

  • Issue of the periodical within the volume

    2199

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    11

  • Pages from-to

    1-11

  • UT code for WoS article

    000644937400007

  • EID of the result in the Scopus database

    2-s2.0-85105905329