Super-resolution fluorescence microscopy by line-scanning with an unmodified two-photon microscope
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F21%3A00345383" target="_blank" >RIV/68407700:21230/21:00345383 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1098/rsta.2020.0300" target="_blank" >https://doi.org/10.1098/rsta.2020.0300</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1098/rsta.2020.0300" target="_blank" >10.1098/rsta.2020.0300</a>
Alternative languages
Result language
angličtina
Original language name
Super-resolution fluorescence microscopy by line-scanning with an unmodified two-photon microscope
Original language description
Fluorescence-based microscopy as one of the standard tools in biomedical research benefits more and more from super-resolution methods, which offer enhanced spatial resolution allowing insights into new biological processes. A typical drawback of utilizing these methods is the need for new, complex optical setups. This becomes even more significant when using two-photon fluorescence excitation, which offers deep tissue imaging and excellent z-sectioning. We show that the generation of striped illumination patterns in twophoton laser scanning microscopy can readily be exploited for achieving optical super-resolution and contrast enhancement utilizing open-source image reconstruction software. The special appeal of this approach is that even in the case of a commercial two-photon laser scanning microscope no optomechanical modifications are required to achieve this modality. Modifying the scanning software with a custom-written macro to address the scanning mirrors in combination with rapid intensity switching by an electro-optic modulator is sufficient to accomplish the acquisition of two-photon striped-illumination patterns on a sCMOS camera. We demonstrate and analyse the resulting resolution improvement by applying different recently published image resolution evaluation procedures to the reconstructed filtered widefield and super-resolved images.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences
ISSN
1364-503X
e-ISSN
1471-2962
Volume of the periodical
379
Issue of the periodical within the volume
2199
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
1-11
UT code for WoS article
000644937400007
EID of the result in the Scopus database
2-s2.0-85105905329