Smallest dual-color qPCR device

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21230%2F23%3A00369124" target="_blank" >RIV/68407700:21230/23:00369124 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/23:10475145 RIV/00064165:_____/23:10475145 RIV/00216305:26220/23:PU149920
Result on the web
<a href="https://doi.org/10.1016/j.snb.2023.134299" target="_blank" >https://doi.org/10.1016/j.snb.2023.134299</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.snb.2023.134299" target="_blank" >10.1016/j.snb.2023.134299</a>

Result language
angličtina
Original language name
Smallest dual-color qPCR device
Original language description
The global outbreak of the coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), necessitated the development of point-of-care (POC) systems for rapid and widespread population screening. Current POC systems, based on reverse transcription–quantitative polymerase chain reaction (RT-qPCR) methods, are unable to perform RNA target multiplexing, which is crucial for specific viral strain identification. This study presents a compact qPCR system capable of dual fluorescence channel gene multiplexing by simultaneously detecting two targets of the SARS-CoV-2 virus using 6-carboxyfluorescein (FAM)- and TxRed-labeled probes. The device measures (110 x 83 x 41) mm3 and weighs 210 gm. It features four virtual reaction chambers, a thermal cycle system, a real-time optical signal detection module, a USB interface, and a Bluetooth wireless data transmission module. Moreover, it can perform single fluorescent channel detection of PCR using intercalating dye, followed by a melting curve analysis and loop-mediated isothermal amplification. The proposed compact dual-color qPCR device significantly broadens its potential for POC applications, such as diagnostics or field testing, molecular biology, microfluidics, and virus detection.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20201 - Electrical and electronic engineering

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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