Using genetic engineering methods for detection lower fungi in digestive tract of primates
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F12%3A00202752" target="_blank" >RIV/68407700:21460/12:00202752 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Using genetic engineering methods for detection lower fungi in digestive tract of primates
Original language description
The aim of this work is detection of eukaryotic organisms-fungi-in digestive tract of primates. The presence of fungi in digestive tract of primates has not been studied yet. The first part of project is to establish appropriate methods of genetic engineering and optimize these methods. Control samples of mouflon faeces are used to establish methodology, because mouflon digestive tract contains fungi, so results of our testing experiments for fungal detection should come outpositive. DNA is isolated from control samples of mouflon faeces, products of DNA amplification by PCR are analyzed by agarose gel electrophoresis.The main goal is setting PCR condition to increase sensitivity of detection of fungal ITS regions. The next step is sequencing and comparison of sequences in the nucleotide database to find typesof fungi. Final testing and experiments at gorilla faeces will take place in future.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/EE.2.3.20.0092" target="_blank" >EE.2.3.20.0092: BIO-XUV Research Team Advancement at FBME CTU</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Instuments and Methods for Biology and Medicine 2012
ISBN
978-80-01-05119-1
ISSN
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e-ISSN
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Number of pages
3
Pages from-to
33-35
Publisher name
Czech Technical University
Place of publication
Prague
Event location
Kladno
Event date
May 29, 2012
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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