Methods of the genetic engineering for mapping of genes that influence response to parasite Leishmania major
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F16%3A00330888" target="_blank" >RIV/68407700:21460/16:00330888 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Methods of the genetic engineering for mapping of genes that influence response to parasite Leishmania major
Original language description
Leishmaniasis is a parasitic disease caused by protozoan of genus Leishmania. These protozoans are transmitted to human body by sandflies (Phlebotomus, Lutzomyia, Psychodopygus) and cause three main forms of pathology: cutaneous, mucocutaneous and visceral. Leishmaniasis is one of the most widespreaddiseases, although is also one of the most neglected diseases in the world, that’s why this issue has different support from WHO. Different species of Leishmania induce duration length and types of manifestation, but even the patients infected by the same species develop different clinical manifestations. Mouse model is frequently used for examination of genetic influence on disease susceptibility, because mouse genome is in 92 % homologous to the human genome. Advance in molecular biology methods, especially genotyping, gave us a tool to study the role of host genotype in a control of several immunological and pathological parameters of leishmaniasis: skin lesion size, splenomegaly, hepatomegaly and parasiteload in organs. We have prepared F2 hybrids between strain OcB-42 and OcB-43, which differ in chromosomal segments on chromosomes 3, 9 and 15. Mice were infected by L. major for 8 weeks and their phenotypes were established. We are currently DNA typing segments on chromosomes 3, 9 and 15. Influence of these segments on tested parameters will be statistically evaluated by ANOVA using Statistica software.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů