Physical stability of small unilamellar vesicles probed by fluorescence correlation spectroscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F17%3A00313968" target="_blank" >RIV/68407700:21460/17:00313968 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Physical stability of small unilamellar vesicles probed by fluorescence correlation spectroscopy
Original language description
Physical stability of model biomembranes in form of vesicles is being investigated since vesicles become a tool for simulations and research of biomembranes and their interactions with the environment or other particles. In this study, we work with small unilamellar vesicles (SUV) composed of phospholipids POPC and DOPS in different ratios and we optimized their preparation protocol. Physical stability of model biomembranes as vesicles can be described as resistance to aggregation and biomembrane disruption. We used fluorescence correlation spectroscopy (FCS) and fluorescence lifetime correlation spectroscopy (FLCS) as suitable techniques to detect these phenomena by observing the diffusion of the fluorescent labels in vesicles. Stability of vesicles was investigated in two steps - first with SUVs labelled by only one dye and next with double-labelled vesicles. Obtained results showed that the stability is dependent on the composition of the vesicles and by adjusting the POPC:DOPS ratio we can achieve up to 6 day stability.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10610 - Biophysics
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Instruments and Methods for Biology and Medicine 2017
ISBN
978-80-01-06303-3
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
1-5
Publisher name
Česká technika - nakladatelství ČVUT, ČVUT v Praze
Place of publication
Praha
Event location
Kladno
Event date
May 5, 2017
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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