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Physical stability of small unilamellar vesicles probed by fluorescence correlation spectroscopy

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F17%3A00313968" target="_blank" >RIV/68407700:21460/17:00313968 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Physical stability of small unilamellar vesicles probed by fluorescence correlation spectroscopy

  • Original language description

    Physical stability of model biomembranes in form of vesicles is being investigated since vesicles become a tool for simulations and research of biomembranes and their interactions with the environment or other particles. In this study, we work with small unilamellar vesicles (SUV) composed of phospholipids POPC and DOPS in different ratios and we optimized their preparation protocol. Physical stability of model biomembranes as vesicles can be described as resistance to aggregation and biomembrane disruption. We used fluorescence correlation spectroscopy (FCS) and fluorescence lifetime correlation spectroscopy (FLCS) as suitable techniques to detect these phenomena by observing the diffusion of the fluorescent labels in vesicles. Stability of vesicles was investigated in two steps - first with SUVs labelled by only one dye and next with double-labelled vesicles. Obtained results showed that the stability is dependent on the composition of the vesicles and by adjusting the POPC:DOPS ratio we can achieve up to 6 day stability.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

  • OECD FORD branch

    10610 - Biophysics

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Instruments and Methods for Biology and Medicine 2017

  • ISBN

    978-80-01-06303-3

  • ISSN

  • e-ISSN

  • Number of pages

    5

  • Pages from-to

    1-5

  • Publisher name

    Česká technika - nakladatelství ČVUT, ČVUT v Praze

  • Place of publication

    Praha

  • Event location

    Kladno

  • Event date

    May 5, 2017

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article