All

What are you looking for?

All
Projects
Results
Organizations

Quick search

  • Projects supported by TA ČR
  • Excellent projects
  • Projects with the highest public support
  • Current projects

Smart search

  • That is how I find a specific +word
  • That is how I leave the -word out of the results
  • “That is how I can find the whole phrase”

Comparison of fluorescence spectra of healthy and carcinoma cell cultures

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F18%3A00326382" target="_blank" >RIV/68407700:21460/18:00326382 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Comparison of fluorescence spectra of healthy and carcinoma cell cultures

  • Original language description

    NADH emission spectra analysis is a promising method of photomedicine to determine the vitality of cells and to obtain information about the metabolic processes in them. The aim of this work is to find the differences in emission spectra between cell cultures of healthy and tumor cells by analyzing the influence of individual contributions from the biofluorophores contained in the cell at the excitation of 260 nm, 340 nm, and 365nm. 260 nm and 340 nm are the excitation maxima of NADH. 365 nm is the excitation wavelength we had found that gives the most intense fluorescence of NADH compares to the rest of biofluorophores. The influence of different cultivation media, glucose adding and oxygen reduction of cell samples on the fluorescence spectra are shown. The experiments were made with the cell cultures of cell line CT26 and 3T3. Measured data were treated by software GASpeD (Genetic Algorithm Spectra Decomposition).

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

  • OECD FORD branch

    20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů