Influence of pH environment on fluorescence spectra of samples of healthy and carcinoma cell cultures

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F19%3A00335623" target="_blank" >RIV/68407700:21460/19:00335623 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Influence of pH environment on fluorescence spectra of samples of healthy and carcinoma cell cultures
Original language description
Influence of pH environment on fluorescence spectra (FS) of NADH is studied on the base of changes these spectra for sample environment pH 5, 7 and 8 and temperature 20 °C. The samples are prepared as suspensions of healthy and carcinoma cell cultures line BALB/3T3 and CT26B in PBS buffer with pH 5, 7, 8 and temperature 20 °C. The normalized FS for excitation wavelength 365 nm were compared for healthy and tumor cells and cannot distinguish between position of the maximum intensity for healthy and tumor cells. The GASpeD program was used to decompose the normalized FS for more detail its analyses. The optimal number of spectra decomposition curves was set at 4. It was found a difference in the concentration of free and bound NADH, with more free than bound NADH in healthy cells, in tumor cells it is the opposite. By comparing normalized FS for the same cells and pH 7, it is assumed that it does not depend on cell concentration at temperature 20 °C. The effect of pH on FS of healthy and tumor cells is the change in concentration of NADH in healthy cells and in concentration of FAD in tumor cells.
Czech name
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Czech description
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Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Instruments and Methods for Biology and Medicine 2019
ISBN
978-80-01-06643-0
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
23-27
Publisher name
Česká technika - nakladatelství ČVUT, ČVUT v Praze
Place of publication
Praha
Event location
Kladno
Event date
May 23, 2019
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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