Melatonin pretreatment does not modify extrasystolic burden in the rat ischemia-reperfusion model

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68407700%3A21460%2F21%3A00351433" target="_blank" >RIV/68407700:21460/21:00351433 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.26402/jpp.2021.1.15" target="_blank" >https://doi.org/10.26402/jpp.2021.1.15</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.26402/jpp.2021.1.15" target="_blank" >10.26402/jpp.2021.1.15</a>

Result language

angličtina

Original language name

Melatonin pretreatment does not modify extrasystolic burden in the rat ischemia-reperfusion model

Original language description

In the present study, we aimed at evaluating the melatonin effects on cardiac sympathetic activity and the incidence of premature ventricular beats during the episode of ischemia-reperfusion. Experiments were done in a total of 26 control and 28 melatonin-treated (10 mg/kg, daily, for 7 days) male rats. Sympathetic fibers density was assessed by glyoxylic acid-induced fluorescence. Continuous electrocardiograms recording was performed during ischemia-reperfusion episodes (5 min/5 min, respectively) induced by reversible coronary occlusion. Myocardial expression of tyrosine hydroxylase, a rate-limiting enzyme of catecholamine biosynthesis was assessed by Western blotting. No differences in the state of sympathetic innervation were observed in histochemical analysis. However, Western blotting analysis demonstrated that melatonin treatment suppressed tyrosine hydroxylase expression in the non-ischemic (p < 0.05 versus control) but not ischemic regions of myocardium. The melatonin-treated animals had longer RR-intervals in the baseline state than the control animals (264 ± 48 ms versus 237 ± 33 ms, p = 0.044, respectively), but this difference decayed during the period of ischemia due to the increase of heart rate in the treated group. The number of premature ventricular beats did not differ between the control and treated groups during the ischemic and reperfusion periods. One-week melatonin pretreatment caused a slight peripheral sympatholytic effect that attenuated during ischemia and completely disappeared by the onset of reperfusion. The slight expression of sympathetic downregulation was associated with the lack of any effect of melatonin on extrasystolic burden. Collectively, the data suggest that melatonin cannot target the triggers of reentrant arrhythmias.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30105 - Physiology (including cytology)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of physiology and pharmacology

ISSN

0867-5910

e-ISSN

1899-1505

Volume of the periodical

72

Issue of the periodical within the volume

1

Country of publishing house

PL - POLAND

Number of pages

8

Pages from-to

141-148

UT code for WoS article

000709404800007

EID of the result in the Scopus database

2-s2.0-85111824283