Using TaqMan Assay Followed By Melting Analysis Performed By New-Generation Intercalating Dyes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70565813%3A_____%2F13%3A%230000290" target="_blank" >RIV/70565813:_____/13:#0000290 - isvavai.cz</a>
Result on the web
<a href="http://www.researchgate.net/publication/249978429_qPCR__NGS_2013_Proceedings_6th_international_qPCR__NGS_Symposium_Industrial_Exhibition__Application_Workshops_Next_Generation_Thinking_in_Molecular_Diagnostics_Editor_Michael_W._Pfaffl_ISBN_9783000410246" target="_blank" >http://www.researchgate.net/publication/249978429_qPCR__NGS_2013_Proceedings_6th_international_qPCR__NGS_Symposium_Industrial_Exhibition__Application_Workshops_Next_Generation_Thinking_in_Molecular_Diagnostics_Editor_Michael_W._Pfaffl_ISBN_9783000410246</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Using TaqMan Assay Followed By Melting Analysis Performed By New-Generation Intercalating Dyes
Original language description
Hydrolysis probes, also known as TaqMan (Applied Biosystems), are routinely used in qPCR diagnostics. The disadvantage of these probes is that they have to be designed for each target sequence separately, which makes the analysis more expensive. In orderto reduce the costs, we can use the UPL probes (Universal ProbeLibrary; Roche), which contain locked nucleic acids and are of short length of just 8-9 nucleotides. The advantage of UPL probes is that they can bind to their targets with increased strength due to the incorporation of locked nucleic acids in their sequence. Their short length allows them to bind to several positions within the whole genome, which could be disadvantageous in some applications. Moreover, the point mutation can lead to the false negative or false positive results due to their short length. This problem can be overcome by using an intercalating dye which has a different fluorescent spectrum than UPL probe and binds unspecifically to any double stranded DNA Th
Czech name
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Czech description
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Project
<a href="/en/project/VF20112015013" target="_blank" >VF20112015013: Research of modern methods of detection and identification of hazardous CBRN substances and materials, methods of hazard reduction and decontamination; research of modern method for personal protection and elements of critical infrastructure</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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