HPLC determination of vitamins B3, B5, and B6 in beef liver

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F09%3A63508764" target="_blank" >RIV/70883521:28110/09:63508764 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

HPLC determination of vitamins B3, B5, and B6 in beef liver

Original language description

Nicotinamide, D-pantothenic acid and pyridoxine are a water soluble light-sensitive vitamins, that were determined in beef liver by HPLC with UV detection. The separation was carried out on a Supelcosil LC 8 column (4,6 x 150 mm, 5?m), using a mobile phase made up to 90% (0.1 mol.dm-3 KH2PO4, pH 7.0) and 10% HPLC grade methanol. The identification was based on the retention time; the quantification was based on the peak area match against those of standard. The extraction process used in our study was based on a combination of acid (0.1 mol.dm-3 HCI) and enzymatic (2% Clara-diastase suspension) hydrolysis. The nicotinamide and D-pantothenic acid content were 2.98 mg.100g-1, 2.8 mg.100g-1 and the pyridoxine content was 3.9 mg.100g-1 in sample. The proposed HPLC method was successfully applied for the determination of B3, B5 and B6 in beef liver.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GM - Food industry

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Fytotechnica et Zootechnica

ISSN

1335-258X

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

SI

Country of publishing house

SK - SLOVAKIA

Number of pages

7

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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