Escherichia coli isolated from food as a risk factor to human health

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F19%3A63523307" target="_blank" >RIV/70883521:28110/19:63523307 - isvavai.cz</a>

Alternative codes found

RIV/70883521:28610/19:63523307

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Escherichia coli isolated from food as a risk factor to human health

Original language description

The Escherichia coli is considered heterogeneous species classified into four main phylogenetic groups A, B1, B2, and D which differ in genome sizes, virulence and antibiotic resistance profiles. To compete with other bacteria,many E. coli strains synthesize antimicrobial agents (colicins, microcins). The ecological role of bacteriocinogeny remains unclear. Whereas commensal strains help maintaining the balance in gastrointestinal tract, pathogenic strains can cause a broad range of human diseases including food poisoning. Objectives: The aim of this study was to investigate the potential threat of Escherichia coli strains isolated from various food to human health. Methods: E. coli strains originating from food (chicken, pork, and game meat, cheese, vegetables, cakes) were phenotypically examined for bacteriocin production and antibiotic resistance (EUCAST), followed by PCR detection of individual bacteriocin, antibiotic resistance, and virulence factor genes. In addition, whole genome sequencing (WGS) was also performed. Results: Altogether, 160 E. coli strains were examined in this study. The most common resistances observed were against aminopenicillins; resistance to fluoroquinolones and aminoglycosides was also prevalent. Among isolated strains, 37% of bacteriocinogeny incidence (colicins: E7, E1, B, M, Ia; microcins: mV, mC7, mB17) was detected. ETEC and EIEC virulence factors were found in game meat and vegetables strains, on the other hand, APEC, UPEC and ExPEC virulence factors were proved in chicken meat and cheese strains. WGS generally confirmed the results detected by PCR. Thus, food can be a source of alimentary infection (game meat, vegetables) and also can be a vehicle of antibiotic resistance spreading.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

21101 - Food and beverages

Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů