Evaluation of genetic diversity of edible honeysuckle monitored by rapd in relation to bioactive substances
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F19%3A63523550" target="_blank" >RIV/70883521:28110/19:63523550 - isvavai.cz</a>
Result on the web
<a href="http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1139/836" target="_blank" >http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1139/836</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5219/1139" target="_blank" >10.5219/1139</a>
Alternative languages
Result language
angličtina
Original language name
Evaluation of genetic diversity of edible honeysuckle monitored by rapd in relation to bioactive substances
Original language description
The aim of this study was clarifying the relation between genetic diversity of edible honeysuckle (Lonicera kamtschatica) and the major group of biologically active substances as total polyphenols content (TPC) including antioxidant activity (AO). Fruits of edible honeysuckle becomes more and more popular, especially in Europe. The current status of research on polyphenolic compounds in the berries of edible honeysuckle and their biological effects, including recommended utilization, are reviewed.The biological material including 14 cultivars of the edible honeysuckle ('Zoluška', 'Amfora', 'Pruhonický 44', 'Vasilijevsky', 'Moskovskaja', 'Vojtek', 'Sinoglaska', 'Altaj', 'Lipnická', 'Kamčadalka', 'Sinaja Ptica', 'Fialka', 'Modrý Triumf', and 'Leningradský velikán') originated from Czech republic (Žabcice near Brno). The content of TPC and AO were determined by location and its soil-climatic conditions and these environmental circumstances determines the RAPD profiles of analysed honeysuckle acessions, too. DPPH method was used to analyze AO and Folin-Ciocalteu method was used to determine TPC. The results of experiment showed that the highest value of AO was determined at the cultivars 'Zoluška' (81.04 mg.L-1) and the lowest was measured in 'Kamčadalka' (54.122 mg.L-1). On the contrary, the highest content of TPC was determined at the cultivar 'Kamčadalka' (51.09 mg.L-1) and the lowest value was measured at the cultivar 'Pruhonický 44' (21.65 mg.L-1). Phylogenetic trees were similar in genetic distance. The content of TPC and AO were not statistically significant in relation to cultivar. The analyzed cultivars of the edible honeysuckle were separated in 4 clusters according to used primers. In both gel images, the amplicon size ranged from 100 to 1,500 bp. We found that genetic diversity was partially related to content of total polyphenolic substances and antioxidant activity. Based on phylogenetic trees we have stated that variety 'Lipnická', 'Sinoglaska', 'Altaj', 'Leningradský velikán', 'Modrý Triumf', 'Sinaja Ptica' and 'Kamčadalka' were grouped in the similar cluster. The highest genetic distance was determined at the variety 'Vasilijevskaja' and 'Amfora'. In the same way, there were variety 'Vojtek', 'Fialka' and 'Zoluška'.
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
21101 - Food and beverages
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Potravinárstvo
ISSN
1338-0230
e-ISSN
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Volume of the periodical
13
Issue of the periodical within the volume
1
Country of publishing house
SK - SLOVAKIA
Number of pages
7
Pages from-to
490-496
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-85067284656