Hierarchically structured polystyrene-based surfaces amplifying fluorescence signals: Cytocompatibility with human induced pluripotent stem cell

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F21%3A63542518" target="_blank" >RIV/70883521:28110/21:63542518 - isvavai.cz</a>
Alternative codes found
RIV/70883521:28610/21:63542518
Result on the web
<a href="https://www.mdpi.com/1422-0067/22/21/11943" target="_blank" >https://www.mdpi.com/1422-0067/22/21/11943</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms222111943" target="_blank" >10.3390/ijms222111943</a>

Result language
angličtina
Original language name
Hierarchically structured polystyrene-based surfaces amplifying fluorescence signals: Cytocompatibility with human induced pluripotent stem cell
Original language description
An innovative multi-step phase separation process was used to prepare tissue culture for the polystyrene-based, hierarchically structured substrates, which mimicked in vivo microenvironment and architecture. Macro-(pore area from 3000 to 18,000 µm2; roughness (Ra) 7.2 ± 0.1 µm) and meso-(pore area from 50 to 300 µm2; Ra 1.1 ± 0.1 µm) structured substrates covered with micro-pores (area around 3 µm2 ) were prepared and characterised. Both types of substrate were suitable for human-induced pluripotent stem cell (hiPSC) cultivation and were found to be beneficial for the induction of cardiomyogenesis in hiPSC. This was confirmed both by the number of promoted proliferated cells and the expressions of specific markers (Nkx2.5, MYH6, MYL2, and MYL7). Moreover, the substrates amplified the fluorescence signal when Ca2+ flow was monitored. This property, together with cytocompatibility, make this material especially suitable for in vitro studies of cell/material interactions within tissue-mimicking environments.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology

Project
<a href="/en/project/GA19-16861S" target="_blank" >GA19-16861S: Interaction of biomaterials with stem cells under in vivo simulated conditions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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