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Structured Surfaces Mimicking in vivo Tissue Architecture

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F22%3A63559998" target="_blank" >RIV/70883521:28610/22:63559998 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Structured Surfaces Mimicking in vivo Tissue Architecture

  • Original language description

    Tissue engineering depends on the availability of appropriate cells seeded on appropriate substrates. Human induced pluripotent stem cells (hiPSC) retain the ability to self-renew and differentiate into any somatic cells and their usage does not invoke any ethical controversy compared to human embryonic stem cells. Another advantage of hiPSC is that the patienťs own cells can be used for therapy and treatment. Therefore, they belong to the most appropriate lineages of tissue engineering testing. Another important aspect in tissue engineering is the kind of substrate on which cells grow. However, there is only limited information available on the impact of surface properties and structures on iPSC behavior. Thus, the influence of a hierarchically structured surface at the level of macro/meso/micro topography on the attachment and maturation of hiPSC-derived cardiomyocytes (hiPSC-CM) was investigated within this study. The structured substrates prepared here mimic the in vivo microenvironment They were tested in the context of their impact on the cardiomyogenesis of humaninduced stem cells. Both types of substrate were suitable for culturing induced cells and, in addition, were found not to inhibit the maturation of cardiomyocytes. The results provided a comprehensive view of the main parameters influencing celUmaterial interactions, with respect to substrate architecture and surface energy. The substrates prepared here offer a new possibility for the experimental set-up ofin vitro experiments.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

  • OECD FORD branch

    20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Result continuities

  • Project

    <a href="/en/project/EF16_028%2F0006243" target="_blank" >EF16_028/0006243: The Development of Capacity for Research and Development of TBU in Zlín</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů