DNA functionalized spider silk nanohydrogels for specific cell attachment and patterning

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F22%3A63554418" target="_blank" >RIV/70883521:28110/22:63554418 - isvavai.cz</a>

Alternative codes found

RIV/70883521:28610/22:63554418

Result on the web

<a href="https://pubs.acs.org/doi/10.1021/acsnano.1c11148" target="_blank" >https://pubs.acs.org/doi/10.1021/acsnano.1c11148</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsnano.1c11148" target="_blank" >10.1021/acsnano.1c11148</a>

Result language

angličtina

Original language name

DNA functionalized spider silk nanohydrogels for specific cell attachment and patterning

Original language description

&quot;Nucleated protein self-assembly of an azido modified spider silk protein was employed in the preparation of nanofibrillar networks with hydrogel-like properties immobilized on coatings of the same protein. Formation of the networks in a mild aqueous environment resulted in thicknesses between 2 and 60 nm, which were controlled only by the protein concentration. Incorporated azido groups in the protein were used to &quot;&quot;click&quot;&quot; short nucleic acid sequences onto the nanofibrils, which were accessible to specific hybridization-based modifications, as proved by fluorescently labeled DNA complements. A lipid modifier was used for efficient incorporation of DNA into the membrane of nonadherent Jurkat cells. Based on the complementarity of the nucleic acids, highly specific DNA-assisted immobilization of the cells on the nanohydrogels with tunable cell densities was possible. Addressability of the DNA cell-to-surface anchor was demonstrated with a competitive oligonucleotide probe, resulting in a rapid release of 75-95% of cells. In addition, we developed a photolithography-based patterning of arbitrarily shaped microwells, which served to spatially define the formation of the nanohydrogels. After detaching the photoresist and PEG-blocking of the surface, DNA-assisted immobilization of the Jurkat cells on the nanohydrogel microstructures was achieved with high fidelity.&quot;

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

21001 - Nano-materials (production and properties)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ACS Nano

ISSN

1936-0851

e-ISSN

1936-086X

Volume of the periodical

16

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

7626-7635

UT code for WoS article

000820339000001

EID of the result in the Scopus database

2-s2.0-85130355739