Novel touchdown-PCR method for the detection of putrescine producing Gram-negative bacteria in food products

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F13%3A43869930" target="_blank" >RIV/70883521:28610/13:43869930 - isvavai.cz</a>
Alternative codes found
RIV/70883521:28110/13:43869930
Result on the web
<a href="http://ac.els-cdn.com/S0740002012002341/1-s2.0-S0740002012002341-main.pdf?_tid=906ed560-acad-11e2-b4d2-00000aacb35e&acdnat=1366787403_27598ec11c61607000a2e1f0584334af" target="_blank" >http://ac.els-cdn.com/S0740002012002341/1-s2.0-S0740002012002341-main.pdf?_tid=906ed560-acad-11e2-b4d2-00000aacb35e&acdnat=1366787403_27598ec11c61607000a2e1f0584334af</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fm.2012.09.024" target="_blank" >10.1016/j.fm.2012.09.024</a>

Result language
angličtina
Original language name
Novel touchdown-PCR method for the detection of putrescine producing Gram-negative bacteria in food products
Original language description
Formation of biogenic amines may occur in food due to metabolic activities of contaminating Gram-negative bacteria. Putrescine is assumed to be the major biogenic amine associated with microbial food spoilage. Gram-negative bacteria can form putrescine by three metabolic pathways that can include eight different enzymes. The objective of this study was to design new sets of primers able to detect all important enzymes involved in the production of putrescine by Gram-negative bacteria. Seven new sets ofconsensual primers based on gene sequences of different bacteria were designed and used for detection of the speA, adiA, adi, speB, aguA, speC, and speF genes. A newly developed touchdown polymerase chain reaction (PCR) method using these primers was successfully applied on several putrescine-producers. Selected PCR products were sequenced and high similarity of their sequences (99-91%) with known sequences of the corresponding genes confirmed high specificity of the developed sets of pr
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GM - Food industry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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