Morphology of Nickel-Chitosan nanoparticles

Result code in IS VaVaI

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Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Morphology of Nickel-Chitosan nanoparticles

Original language description

Chitosan is a polysaccharide and derivate of chitin. From a chemical viewpoint, is a linear copolymer composed of randomly distributed β(1-4) N-acetyl glucosamine. It dissolves only under acidic conditions because of its pKa value (6.5). It is widely used in pharmaceutical and biomedical applications because of its unique properties, such as biocompatibility, biodegradability, antibacterial activity, and non-toxicity. Chitosan nanoparticles (CSNPs) can be prepared by various techniques such as emulsion cross linking, reverse micellar method, linking spray drying, and ion-gelation method. Ion-gelation method is based on electrostatic interactions between positive and negative charges. Chitosan is a cationic polymer that can stabilize anionic substances under acidic conditions. In this case, the negative charge of polyanion - sodium tripolyphosphate (TPP) was used. TPP was continuously dropped to the chitosan solution (pH = 4.8) and stirred for 30 minutes. Thereafter, two opposite charges will combine based on physical crosslinking to chitosan nanocarriers. Prepared chitosan nanoparticles were modified with solution of 0.06 M NiCl2 . 6 H2O. Nickel solution (200 µL) was slowly dropped into the colloidal system of chitosan nanoparticles. The prepared colloidal system was centrifugated at 10 000 rpm for 15 minutes. Chitosan nanoparticles modified with nickel (Ni-CSNPs) have potential applications in electrochemical insulin sensors. Based on previous research, nickel or nickel oxide nanoparticles could be considered great candidates for electrochemical insulin sensors. Nickel shows catalytic activity at alkaline pH by creating the specific electrocatalytic anion NiOOH-. The presence of NiOOH − anions is essential for direct and rapid electrochemical insulin oxidation. The morphology of the prepared Ni-CSNPs was studied using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Based on the obtained results, spherical structures were observed with the average size of 158 nm calculated from the TEM image. Size of CSNPs based on TEM images is shown in histogram. TEM image shows black dost, which indicates that nickel have been attached to the surface of chitosan nanoparticles. The EDX analysis obtained from the red area of the SEM image indicated 7.24 wt. % nickel, 29.1 wt% of carbon, 36.3 wt.% of oxygen and less than 1 wt.% of chlorine. The presence of chlorine is caused by the addition of nickel chloride to the already-formed chitosan nanoparticles. Based on results of the microscopic methods and EDX analysis, the surfaces of the chitosan nanoparticles were successfully modified by nickel. Electrodes modified with Ni-CSNPs show potential as electrochemical sensors for direct and fast insulin oxidation. The development of insulin electrochemical sensors is necessary because of the worldwide increase in the number of people suffering from diabetes mellitus.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů