Origin in Acinetobacter gyllenbergii and dissemination of aminoglycoside-modifying enzyme AAC(6')-Ih
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F16%3A00011314" target="_blank" >RIV/75010330:_____/16:00011314 - isvavai.cz</a>
Result on the web
<a href="http://jac.oxfordjournals.org/content/71/3/601" target="_blank" >http://jac.oxfordjournals.org/content/71/3/601</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jac/dkv390" target="_blank" >10.1093/jac/dkv390</a>
Alternative languages
Result language
angličtina
Original language name
Origin in Acinetobacter gyllenbergii and dissemination of aminoglycoside-modifying enzyme AAC(6')-Ih
Original language description
The aac(6')-Ih gene encoding aminoglycoside 6'-N-acetyltransferase type I subtype h [AAC(6')-Ih] is plasmid-borne in Acinetobacter baumannii where it confers high-level amikacin resistance, but its origin remains unknown. We searched for the gene in the genomes of a collection of 133 Acinetobacter spp. and studied its species specificity, expression and dissemination. Gene copy number was determined by quantitative PCR, expression by quantitative RT-PCR, MIC by microdilution and transfer by plasmid mobilization. The aac(6')-Ih gene was present in the chromosome of the two Acinetobacter gyllenbergii of the collection and was detected in all seven A. gyllenbergii clinical isolates. They had indistinguishable flanking regions indicating that the gene was intrinsic to this species. A. baumannii P-ISAba23 promoters were provided by insertion of ISAba23, which disrupted the P-native promoter in A. gyllenbergii. Both types of promoters were similarly potent in Escherichia coli and A. baumannii. Aminoglycoside MICs for A. baumannii harbouring pIP1858 were higher than for A. gyllenbergii due to gene dosage. The non-self-transferable plasmid could be mobilized to other A. baumannii cells by the broad host range plasmid RP4. We have found the origin of aac(6')-Ih in A. gyllenbergii, a species isolated, although rarely, in humans, and documented that dissemination of this gene is restricted to the Acinetobacter genus.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NT14466" target="_blank" >NT14466: Genomic analysis of antibiotic multiresistance in a global epidemic clone of Acinetobacter baumannii.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Antimicrobial Chemotherapy
ISSN
0305-7453
e-ISSN
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Volume of the periodical
71
Issue of the periodical within the volume
3
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
601-606
UT code for WoS article
000372984200007
EID of the result in the Scopus database
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