TEM-1 beta-lactamase as a source of resistance to sulbactam in clinical strains of Acinetobacter baumannii
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10159322" target="_blank" >RIV/00216208:11310/13:10159322 - isvavai.cz</a>
Result on the web
<a href="http://jac.oxfordjournals.org/content/68/12/2786" target="_blank" >http://jac.oxfordjournals.org/content/68/12/2786</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/jac/dkt275" target="_blank" >10.1093/jac/dkt275</a>
Alternative languages
Result language
angličtina
Original language name
TEM-1 beta-lactamase as a source of resistance to sulbactam in clinical strains of Acinetobacter baumannii
Original language description
Objectives: Sulbactam is well known to have clinically relevant intrinsic activity against Acinetobacter baumannii. Although secondary resistance to this drug has long been reported in acinetobacters, virtually nothing is known about its molecular basis.The aim of this study was to test the hypothesis that beta-lactamase TEM-1 is responsible for sulbactam resistance in A. baumannii. Methods: Seventeen clinical strains of A. baumannii were selected to represent different combinations of quantitative susceptibilities to sulbactam and molecular typing characteristics. The strains were screened by PCR for the presence of the bla(TEM-1) gene and its variants. Amplicons encompassing the bla(TEM) genes, including their promoters, were sequenced. The expression and copy number of the bla(TEM) genes were assessed using semi-quantitative real-time PCR. Transfer of the bla(TEM-1) gene into a susceptible A. baumannii strain was achieved by electroporation. Results: Six strains were negative for t
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FN - Epidemiology, infection diseases and clinical immunology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Antimicrobial Chemotherapy
ISSN
0305-7453
e-ISSN
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Volume of the periodical
68
Issue of the periodical within the volume
12
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
2786-2791
UT code for WoS article
000326980000011
EID of the result in the Scopus database
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