Insights into the Resistome and Phylogenomics of a ST195 Multidrug-Resistant Acinetobacter baumannii Clinical Isolate from the Czech Republic
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F21%3A00075298" target="_blank" >RIV/65269705:_____/21:00075298 - isvavai.cz</a>
Alternative codes found
RIV/00098892:_____/21:N0000014 RIV/62157124:16810/21:43879648 RIV/62157124:16270/21:43879648 RIV/61989592:15110/21:73612646
Result on the web
<a href="https://www.mdpi.com/2075-1729/11/10/1079" target="_blank" >https://www.mdpi.com/2075-1729/11/10/1079</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/life11101079" target="_blank" >10.3390/life11101079</a>
Alternative languages
Result language
angličtina
Original language name
Insights into the Resistome and Phylogenomics of a ST195 Multidrug-Resistant Acinetobacter baumannii Clinical Isolate from the Czech Republic
Original language description
Increasing antimicrobial resistance in nosocomial pathogens, such as Acinetobacter baumannii, is becoming a serious threat to public health. It is necessary to detect beta-lactamase-producing microorganisms in clinical settings to be able to control the spread of carbapenem resistance. This study was conducted to evaluate the presence of beta-lactamases in a selected clinical isolate of A. baumannii of ST2(P)/ST195(Ox) and to characterize possible enzymes, as well as its beta-lactam resistome, using PCR and whole-genome sequencing analysis. PCR and sequencing confirmed that the isolate harbored five bla gene alleles, namely, bla(ADC-73), bla(TEM-1), bla(OXA-23), bla(OXA-58) and bla(OXA-66), as well as aminoglycosides, macrolides, sulfonamides and tetracyclines resistance determinants, which were either chromosomally and/or plasmid located. Furthermore, a gene order comparison using MAUVE alignment showed multiple changes compared with the clinical isolate of Malaysian A. baumannii AC30 genome and 76 regions with high homology. This study suggests that resistance to beta-lactams in this A. baumannii isolate is mainly due to an overproduction of beta-lactamases in combination with other resistance mechanism (efflux pump system).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/NU20J-09-00040" target="_blank" >NU20J-09-00040: Whole genome sequencing and metagenomics towards understanding transmission routes of antibiotic resistant bacteria from hospitals to the environment</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Life-Basel
ISSN
2075-1729
e-ISSN
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Volume of the periodical
11
Issue of the periodical within the volume
10
Country of publishing house
CH - SWITZERLAND
Number of pages
14
Pages from-to
1079
UT code for WoS article
000714615600001
EID of the result in the Scopus database
2-s2.0-85117712968