Structure and topology around the cleavage site regulate post-translational cleavage of the HIV-1 gp160 signal peptide

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F17%3A00011797" target="_blank" >RIV/75010330:_____/17:00011797 - isvavai.cz</a>

Result on the web

<a href="https://elifesciences.org/articles/26067" target="_blank" >https://elifesciences.org/articles/26067</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.7554/eLife.26067" target="_blank" >10.7554/eLife.26067</a>

Result language

angličtina

Original language name

Structure and topology around the cleavage site regulate post-translational cleavage of the HIV-1 gp160 signal peptide

Original language description

Like all other secretory proteins, the HIV-1 envelope glycoprotein gp160 is targeted to the endoplasmic reticulum (ER) by its signal peptide during synthesis. Proper gp160 folding in the ER requires core glycosylation, disulfide-bond formation and proline isomerization. Signal-peptide cleavage occurs only late after gp160 chain termination and is dependent on folding of the soluble subunit gp120 to a near-native conformation. We here detail the mechanism by which co-translational signal-peptide cleavage is prevented. Conserved residues from the signal peptide and residues downstream of the canonical cleavage site form an extended alpha-helix in the ER membrane, which covers the cleavage site, thus preventing cleavage. A point mutation in the signal peptide breaks the alpha helix allowing co-translational cleavage. We demonstrate that postponed cleavage of gp160 enhances functional folding of the molecule. The change to early cleavage results in decreased viral fitness compared to wild-type HIV.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30303 - Infectious Diseases

Project

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Continuities

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

eLIFE

ISSN

2050-084X

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

July

Country of publishing house

GB - UNITED KINGDOM

Number of pages

25

Pages from-to

nestrankovano

UT code for WoS article

000408198100001

EID of the result in the Scopus database

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