Effects of proteases from pineapple and papaya on protein digestive capacity and gut microbiota in healthy C57BL/6 mice and dose-manner response on mucosal permeability in human reconstructed intestinal 3D tissue model

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F22%3A00014086" target="_blank" >RIV/75010330:_____/22:00014086 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2218-1989/12/11/1027" target="_blank" >https://www.mdpi.com/2218-1989/12/11/1027</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/metabo12111027" target="_blank" >10.3390/metabo12111027</a>

Result language

angličtina

Original language name

Effects of proteases from pineapple and papaya on protein digestive capacity and gut microbiota in healthy C57BL/6 mice and dose-manner response on mucosal permeability in human reconstructed intestinal 3D tissue model

Original language description

Cysteine proteases obtained from the stem of pineapple or papaya latex, bromelain and papain, respectively, exhibit a broad spectrum of beneficial effects on human health. However, their effects on gut microbiota composition or dose-manner effects on the intestinal integrity of healthy tissue have not been evaluated. In this study, C57BL/6 young, healthy mice were fed bromelain or papain in a dose of 1 mg per animal/day for three consecutive days, followed by the assessment of digestive protein capacity, intestinal morphology and gut microbiota composition. Furthermore, a human reconstructed 3D tissue model Epilntestinal (SMI-100) was used to study the effects of 1, 0.1 and 10 mg/mL doses of each enzyme on tissue integrity and mucosal permeability using TEER measurements and passage of Lucifer Yellow marker from the apical to the basolateral side of the mucosa. The results indicated that fruit proteases have the potential to modulate gut microbiota with decreasing abundance of Proteobacteria and increasing beneficial Akkermansia muciniphila. The enhancement of pancreatic trypsin was observed in bromelain and papain supplementation, while bromelain also increased the thickness of the ileal mucosa. Furthermore, an in vitro study showed a dose-dependent interruption in epithelial integrity, which resulted in increased paracellular permeability by the highest doses of enzymes. These findings define bromelain and papain as promising enzymatic supplementation for controlled enhancement of paracellular uptake when needed, together with beneficial effects on the gut microbiota.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30108 - Toxicology

Project

<a href="/en/project/EF16_019%2F000860" target="_blank" >EF16_019/000860: International competitiveness of NIPH in research, development and education in alternative toxicological methods.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Metabolites

ISSN

2218-1989

e-ISSN

2218-1989

Volume of the periodical

12

Issue of the periodical within the volume

11

Country of publishing house

CH - SWITZERLAND

Number of pages

15

Pages from-to

1027

UT code for WoS article

000881208100001

EID of the result in the Scopus database

2-s2.0-85141788087