Investigation of 29 antimicrobial compounds in soil using newly developed UHPLC-MS/MS method

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F23%3A00014395" target="_blank" >RIV/75010330:_____/23:00014395 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/1420-3049/28/18/6496" target="_blank" >https://www.mdpi.com/1420-3049/28/18/6496</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/molecules28186496" target="_blank" >10.3390/molecules28186496</a>

Result language

angličtina

Original language name

Investigation of 29 antimicrobial compounds in soil using newly developed UHPLC-MS/MS method

Original language description

While the prudent and reasonable use of veterinary antimicrobial agents in food-producing animals is necessary, researchers over the decades have shown that these antimicrobial agents can spread into the environment through livestock manure and wastewater. The analysis of the occurrence of antimicrobial compounds in soil samples is of a great importance to determine potential impacts on human and animal health and the environment. In this study, an affordable, rugged and simple analytical method has been developed for the determination of twenty-nine antimicrobial compounds from five different classes (tetracyclines, fluoro(quinolones), macrolides, sulfonamides and diaminopirimidines). Liquid-liquid extraction (LLE) with extract filtration combined with ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was the best strategy for the simultaneous determination of all analytes. The developed method was validated according to the Commission Implementing Regulation (EU) 2021/808. The limit of detections (LODs) ranged from 0.5 to 2.0 mu g/kg, while the limit of quantitation (LOQ) was established at 1.0 to 20.0 mu g/kg. The developed method was successfully applied for the determination of antimicrobial residues in one hundred and eighteen soil samples obtained from four European countries (Austria, Czech Republic, Estonia and Portugal). Doxycycline in the concentration levels of 9.07 mu g/kg-20.6 mu g/kg was detected in eight of the analysed samples. Samples were collected from areas where natural fertilizers (swine or cow manure) were applied. Our method can be efficiently used to monitor anti-microbial compounds in soil samples.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30304 - Public and environmental health

Project

—

Continuities

R - Projekt Ramcoveho programu EK

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecules

ISSN

1420-3049

e-ISSN

1420-3049

Volume of the periodical

28

Issue of the periodical within the volume

18

Country of publishing house

CH - SWITZERLAND

Number of pages

14

Pages from-to

6496

UT code for WoS article

001075061900001

EID of the result in the Scopus database

2-s2.0-85172777366