Genotyping of European Toxoplasma gondii strains by a new high-resolution next-generation sequencing-based method
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F75010330%3A_____%2F24%3A00014808" target="_blank" >RIV/75010330:_____/24:00014808 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16170/24:43880571 RIV/62157124:16810/24:43880571
Result on the web
<a href="https://link.springer.com/article/10.1007/s10096-023-04721-7" target="_blank" >https://link.springer.com/article/10.1007/s10096-023-04721-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10096-023-04721-7" target="_blank" >10.1007/s10096-023-04721-7</a>
Alternative languages
Result language
angličtina
Original language name
Genotyping of European Toxoplasma gondii strains by a new high-resolution next-generation sequencing-based method
Original language description
Purpose A new high-resolution next-generation sequencing (NGS)-based method was established to type closely related European type II Toxoplasma gondii strains.Methods T. gondii field isolates were collected from different parts of Europe and assessed by whole genome sequencing (WGS). In comparison to ME49 (a type II reference strain), highly polymorphic regions (HPRs) were identified, showing a considerable number of single nucleotide polymorphisms (SNPs). After confirmation by Sanger sequencing, 18 HPRs were used to design a primer panel for multiplex PCR to establish a multilocus Ion AmpliSeq typing method. Toxoplasma gondii isolates and T. gondii present in clinical samples were typed with the new method. The sensitivity of the method was tested with serially diluted reference DNA samples.Results Among type II specimens, the method could differentiate the same number of haplotypes as the reference standard, microsatellite (MS) typing. Passages of the same isolates and specimens originating from abortion outbreaks were identified as identical. In addition, seven different genotypes, two atypical and two recombinant specimens were clearly distinguished from each other by the method. Furthermore, almost all SNPs detected by the Ion AmpliSeq method corresponded to those expected based on WGS. By testing serially diluted DNA samples, the method exhibited a similar analytical sensitivity as MS typing.Conclusion The new method can distinguish different T. gondii genotypes and detect intra-genotype variability among European type II T. gondii strains. Furthermore, with WGS data additional target regions can be added to the method to potentially increase typing resolution.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30310 - Parasitology
Result continuities
Project
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Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal of Clinical Microbiology and Infectious Diseases
ISSN
0934-9723
e-ISSN
1435-4373
Volume of the periodical
43
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
355-371
UT code for WoS article
001129755400001
EID of the result in the Scopus database
2-s2.0-85179934861