A novel Ion AMpliSeq-based typing method of Toxoplasma gondii reveals aN ExCELLENT typing resolution among European type II strains
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F22%3A43880143" target="_blank" >RIV/62157124:16170/22:43880143 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/22:43880143
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
A novel Ion AMpliSeq-based typing method of Toxoplasma gondii reveals aN ExCELLENT typing resolution among European type II strains
Original language description
Toxoplasma gondii has a clonal population structure in Europe. The currently available typing method, based on 15 microsatellite regions, has a limited ability to discriminate between different type II strains. We therefore aimed to establish a next-generation sequencing (NGS)-based method with a higher typing resolution.Methods: T. gondii isolates were collected from different parts of Europe and assessed by whole genome sequencing (WGS). Highly polymorphic regions (HPRs) were identified. After confirmation by Sanger sequencing, 18 HPRs were used to design a primer panel suitable to establish an Ion AmpliSeq-based typing tool.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů