Human seminal plasma proteins on sperm surface detected by monoclonal antibodies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F09%3A00326897" target="_blank" >RIV/86652036:_____/09:00326897 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Human seminal plasma proteins on sperm surface detected by monoclonal antibodies
Original language description
We have generated antibodies to sperm and in our panel of mouse monoclonal antibodies to human spermatozoa several antibodies - Hs-3, Hs-16, Hs-23, Hs-9, Hs-31, Hs-32 and Hs-37 - reacted with human seminal plasma. These antibodies were characterized in detail using indirect immunofluorescence, Western blot techniques and MALDI TOF sequence analysis and recognized apolipoprotein J (clusterin), semenogelin I, secretory actin-binding protein (SABP) and enolase I. Proteins detected by our monoclonal antibodies can be useful in investigation of the mechanisms involved in sperm pathology and also represent candidates for diagnostic markers during sperm quality assessement. Further experiments concerning the role of above mentioned proteins in capacitation, acrosomal reaction and sperm-egg binding are in progress.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
DN - Environmental impact on health
OECD FORD branch
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Result continuities
Project
<a href="/en/project/1M06011" target="_blank" >1M06011: The Centre of Molecular Methods for Monitoring the Diffuse Pollution of the Environment.</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů