Study of acrosin inhibitor along the boar epididymis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F10%3A00349095" target="_blank" >RIV/86652036:_____/10:00349095 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Study of acrosin inhibitor along the boar epididymis
Original language description
Sperm maturation in the epididymis represents a key step in the reproduction process. Sperm plasma membrane is exposed to epididymal fluid representing the natural environment essential for their post-testicular maturation. Changes in the sperm membraneproteins are influenced by proteolytic and glycosidic enzymes present in the fluid. We monitored acrosin inhibitor (AI) in epididymal fluid and spermatozoa along the organ. Boar epididymal fluid with spermatozoa was isolated according to its spatial restriction into 9 fractions. The AI was detected in sperm extracts and fluids using specific polyclonal antibody. Expression of AI gene was investigated by PCR method. Additionally, immunofluorescence technique (IMF) was used for localization in the tissue.We registered the increasing signal of AI from caput to cauda epididymis. We confirmed that using gene expression method. Using specific antibody, AI was localized in the secretory tissues of caput, corpus and cauda epididymis by IMF.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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