Increasing Affinity of Interferon-gamma Receptor 1 to Interferon-gamma by Computer-Aided Design

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F13%3A00420825" target="_blank" >RIV/86652036:_____/13:00420825 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1155/2013/752514" target="_blank" >http://dx.doi.org/10.1155/2013/752514</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2013/752514" target="_blank" >10.1155/2013/752514</a>

Result language

angličtina

Original language name

Increasing Affinity of Interferon-gamma Receptor 1 to Interferon-gamma by Computer-Aided Design

Original language description

We describe a computer-based protocol to design protein mutations increasing binding affinity between ligand and its receptor. The method was applied to mutate interferon-gamma receptor 1 (IFN-gamma-Rx) to increase its affinity to natural ligand IFN-gamma, protein important for innate immunity. We analyzed all four available crystal structures of the IFN-gamma-Rx/IFN-gamma complex to identify 40 receptor residues forming the interface with IFN-gamma. For these 40 residues, we performed computational mutation analysis by substituting each of the interface receptor residues by the remaining standard amino acids. The corresponding changes of the free energy were calculated by a protocol consisting of FoldX and molecular dynamics calculations. Based on thecomputed changes of the free energy and on sequence conservation criteria obtained by the analysis of 32 receptor sequences from 19 different species, we selected 14 receptor variants predicted to increase the receptor affinity to IFN-ga

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

<a href="/en/project/GAP305%2F10%2F2184" target="_blank" >GAP305/10/2184: Structure-function relationships underlying protein-protein interactions</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BIOMED RESEARCH INTERNATIONAL

ISSN

2314-6133

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

752514

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

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UT code for WoS article

000325638500001

EID of the result in the Scopus database

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