Novel high-affinity binders of human interferon gamma derived from albumin-binding domain of protein G
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F12%3A00385253" target="_blank" >RIV/61388971:_____/12:00385253 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/12:00385253 RIV/44555601:13440/12:43883912 RIV/67985882:_____/12:00385253
Result on the web
<a href="http://dx.doi.org/10.1002/prot.23234" target="_blank" >http://dx.doi.org/10.1002/prot.23234</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/prot.23234" target="_blank" >10.1002/prot.23234</a>
Alternative languages
Result language
angličtina
Original language name
Novel high-affinity binders of human interferon gamma derived from albumin-binding domain of protein G
Original language description
Recombinant ligands derived from small protein scaffolds show promise as robust research and diagnostic reagents and next generation protein therapeutics. Here, we derived high-affinity binders of human interferon gamma (hIFN gamma) from the three helixbundle scaffold of the albumin-binding domain (ABD) of protein G from Streptococcus G148. Computational interaction energy mapping, solvent accessibility assessment, and in silico alanine scanning identified 11 residues from the albumin-binding surface of ABD as suitable for randomization. A corresponding combinatorial ABD scaffold library was synthesized and screened for hIFN gamma binders using in vitro ribosome display selection, to yield recombinant ligands that exhibited Kd values for hIFN gamma from 0.2 to 10 nM
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Proteins-Structure, Function and Bioinformatics
ISSN
0887-3585
e-ISSN
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Volume of the periodical
80
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
774-789
UT code for WoS article
000300053500026
EID of the result in the Scopus database
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